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Title   p53 유전자의 인체 대장 세포주 이입에 대한 세포 생물학적 효과 ( Effects of Wild - type p53 Gene Transfection into Human Colon Cancer Cell Line )
Publicationinfo   1999 Jan; 031(02): 367-377.
Key_word   Colon cancer celi lines, p53 tumor suppressor gene, Liposome, Transfection
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Abstract   Purpose: In colon cancer, the most frequent genetic alteration is found in p53 tumor suppressor gene residing on the short arm of chromosome 17. In order to investigate the significance of wild-type p53, we transfected wild type p53 into human colon cancer cell lines and analysed tbeir biologic effects. Materials and Methods: For analysis of p53 status in cell lines, polymerase chain reaction-single stranded confonnation polymorphism (PCR-SSCP), PCR-direct sequencing and Western blot analysis were employed. Transient transfection with liposome-p53 complex was followed by cell biologic assay. Results: We found that twelve of fifteen human colon cancer cell lines showed mutation of p53 by PCR-SSCP method. These results almost corresponded to p53 protein accumulations assessed by Westem blot using PAbl801. After transfection with lipafect- AMINE and wild type p53 complex on p53 mutant type cell line (LS1034), viability was reduced to 17.9%, and invasiveness was reduced to 37.3%. Morphologically, wild type p53 transfected cells showed lumen formation and apoptosis after induction of differ- entiation by Matrigel. Conclusion: Wild type p53 transfection into p53 mutated colon cancer ceil line resulted in restoration of tumor suppressor effect of p53, and this model would be one of the experimental systems for p53-based gene therapy.
저 자   김현옥(Hyun Ok Kim),김우호(Woo Ho Kim),배수인(Soo In Bae),이혜원(He Won Lee),김종재(Chong Jai Kim),홍성렬(Sung Youl Hong),김용일(Yong Il Kim)